Jul 1, 2007 · The composition of the cow dung is shown in Table 1. After it was slowly thawed, the cow dung (200 g) was placed in a 625 ml glass bottle. The bottle was tightly sealed with a butyl rubber cap after replacement of the gas phase with nitrogen. The cow dung was statically cultured in an incubator at 37°C, 47°C, 55°C, 60°C, 67°C, and 75°C