Mar 1, 2014 · In tanks H1, H2, H3 and H4, the proportions of these bacteria increased by 4.98, 4.48, 19.8, and 3.9 times, respectively. The proportional increase in this family of bacteria was found to be the largest in fermentation tank H3 (mass to volume ratio of PM to MAW 1:1), suggesting that this ratio is most conducive to methane production.